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Image Search Results
Journal: Applied Microbiology and Biotechnology
Article Title: CRISPR/Cas9 genome editing technology in filamentous fungi: progress and perspective
doi: 10.1007/s00253-019-10007-w
Figure Lengend Snippet: Schematic illustration of Cas9/gRNA genome editing. a sgRNA-mediated Cas9 protein can bind to target sequences of site and cut DNA double strands. b When the DNA double-strand breaks occur, the cell initiates the self-repair mechanism. The NHEJ-dominated repair pathway will cause the random loss, insertion, and replacement of bases at the breakage point, resulting in gene mutation. The HR pathway will accurately edit the target gene guided by the donor DNA fragment. c Single-promoter-driven gRNA expression cassette. d multiple gRNA expression cassettes can be constructed by concatenating 2 or more of gRNA linked together by linkers, which can then be enzymatically processed into multiple single gRNAs thus targeting multiple sites
Article Snippet: L. maculans ,
Techniques: Mutagenesis, Expressing, Construct
Journal: Applied Microbiology and Biotechnology
Article Title: CRISPR/Cas9 genome editing technology in filamentous fungi: progress and perspective
doi: 10.1007/s00253-019-10007-w
Figure Lengend Snippet: The history of the development and application of CRISPR/Cas9 technology in filamentous fungi; different colors represent different promoter-driven gRNA expression cassettes
Article Snippet: L. maculans ,
Techniques: CRISPR, Expressing
Journal: Applied Microbiology and Biotechnology
Article Title: CRISPR/Cas9 genome editing technology in filamentous fungi: progress and perspective
doi: 10.1007/s00253-019-10007-w
Figure Lengend Snippet: The application of the CRISPR/Cas9 system in filamentous fungi
Article Snippet: L. maculans ,
Techniques: CRISPR, Expressing, Selection, Marker, Disruption, Modification, Electroporation